| First Author | Fukaya Takashi | Year | 2017 |
| Journal | Curr Biol | Volume | 27 |
| Pages | 1387-1391 | PubMed ID | 28457866 |
| Abstract Text | Elongation of RNA polymerase II (Pol II) is thought to be an important mechanism for regulating gene expression [1]. We measured the first wave of de novo transcription in living Drosophila embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA stem loops. Pol II elongation rates of 2.4-3.0 kb/min were observed, approximately twice as fast as earlier estimates [2-6]. The revised rates permit substantial levels of zygotic gene activity prior to the mid-blastula transition. We also provide evidence that variable rates of elongation are not a significant source of differential gene activity, suggesting that transcription initiation and Pol II release are the key determinants of gene control in development. | Doi | 10.1016/j.cub.2017.03.069 |
| Issue | 9 | Month | May |
