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Publication : Structural and functional analysis of single neurons to correlate synaptic connectivity with grooming behavior.

First Author  Kays Ibrahim Year  2014
Journal  Nat Protoc Volume  9
Pages  1-10 PubMed ID  24309972
Abstract Text  We describe a protocol to image the complex axonal branching structure of identified mechanosensory neurons in Drosophila, combined with a behavioral assay to evaluate the functional output of the neuron. The stimulation of identified mechanosensory neurons in live animals produces a stereotyped grooming reflex. The mechanosensory axonal arbor within the CNS is subsequently labeled with a lipophilic fluorescent dye and imaged by fluorescence microscopy. The behavioral output can therefore be correlated to the axonal morphology of the stimulated neuron in the same animal. Combining this protocol with genetic analysis provides a powerful tool for identifying the roles of molecules involved in different aspects of hard-wired neural circuit formation underlying an innate behavior. From behavioral analysis to axonal imaging, the protocol takes 4 d. Doi  10.1038/nprot.2013.157
Issue  1 Month  Jan

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