help  | about  | cite  | software

Publication : MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring.

First Author  Jankovics Ferenc Year  2002
Journal  Curr. Biol. Volume  12
Pages  2060-5 PubMed ID  12477397
Abstract Text  In Drosophila, development of the embryonic germ cells depends on posterior transport and site-specific translation of oskar (osk) mRNA and on interdependent anchoring of the osk mRNA and protein within the posterior subcortical region of the oocyte. Transport of the osk mRNA is mediated by microtubules, while anchoring of the osk gene products at the posterior pole of the oocyte is suggested to be microfilament dependent. To date, only a single actin binding protein (TropomyosinII) has been identified with a putative role in osk mRNA and protein anchoring. This communication demonstrates that mutations in the Drosophila moesin (Dmoe) gene that encodes another actin binding protein result in delocalization of osk mRNA and protein from the posterior subcortical region and, as a consequence, in failure of embryonic germ cell development. In Dmoe mutant oocytes, the subcortical actin network is detached from the cell membrane, while the polarized microtubule cytoskeleton is unaffected. In line with the earlier observations, colocalization of ectopic actin and OSK protein in Dmoe mutants suggests that the actin cytoskeleton anchors OSK protein to the subcortical cytoplasmic area of the Drosophila oocyte. Doi  10.1016/s0960-9822(02)01256-3
Issue  23 Month  Dec

Publication Annotations Displayer

26 Entities

16 Mesh Terms