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Publication : Novel DNA-binding protein from Drosophila embryos identified by binding site selection.

First Author  Todorović V Year  1996
Journal  FEBS Lett. Volume  396
Pages  99-102 PubMed ID  8906875
Abstract Text  The DNA sequence C/AGAGCGC/AGA, related to binding sites for GAF and Zeste transcription factors, was selected from a pool of degenerate PCR fragments for binding to the cytoplasmic protein of Drosophila preblastoderm embryos. Identical DNA binding activity was also detected in embryonic nuclei. Based on several criteria, such as size, intracellular distribution, sensitivity to ATP and protein kinase inhibitor 6-DMAP, kinetics during development and lack of cross-reaction with rabbit anti-GAF serum, protein recognizing selected sequence was shown to differ from either Zeste or GAF. Doi  10.1016/0014-5793(96)01078-2
Issue  1 Month  Oct

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