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Publication : Genetic analysis of myoblast fusion: blown fuse is required for progression beyond the prefusion complex.

First Author  Doberstein S K Year  1997
Journal  J. Cell Biol. Volume  136
Pages  1249-61 PubMed ID  9087441
Abstract Text  The events of myoblast fusion in Drosophila are dissected here by combining genetic analysis with light and electron microscopy. We describe a new and essential intermediate step in the process, the formation of a prefusion complex consisting of "paired vesicles."These pairs of vesicles from different cells align with each other across apposed plasma membranes. This prefusion complex resolves into dense membrane plaques between apposed cells; these cells then establish cytoplasmic continuity by fusion of small areas of plasma membrane followed by vesiculation of apposed membranes. Different steps in this process are specifically blocked by mutations in four genes required for myoblast fusion. One of these genes, blown fuse, encodes a novel cytoplasmic protein expressed in unfused myoblasts that is essential for progression beyond the prefusion complex stage. Doi  10.1083/jcb.136.6.1249
Issue  6 Month  Mar

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