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Publication : Arthrin, a myofibrillar protein of insect flight muscle, is an actin-ubiquitin conjugate.

First Author  Ball E Year  1987
Journal  Cell Volume  51
Pages  221-8 PubMed ID  2822254
Abstract Text  Flight muscles of some insects contain a myofibrillar protein termed arthrin, which is closely related to actin (mw 43,000). Here we demonstrate that arthrin (mw 55,000) is ubiquitinated actin. We show that in Act88FM342, a flightless Drosophila mutant wherein the Act88F actin gene specifies a glu93----lys replacement, isoelectric points of both actin III and arthrin are shifted, revealing that both are encoded by the same gene. Arthrin reacts with an anti-ubiquitin antibody, which demonstrates that its extra mass results from ubiquitin ligation. Approximately one-seventh of myofibrillar actin is stably ubiquitinated, suggesting that there may be one arthrin molecule per actin-tropomyosin-troponin cooperative unit. Arthrin formation lags several hours behind that of actin III, implying that ubiquitination coincides with some aspect of myofibril assembly. Doi  10.1016/0092-8674(87)90149-8
Issue  2 Month  Oct

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