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Publication : Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis.

First Author  Reichholf Brian Year  2019
Journal  Mol. Cell Volume  75
Pages  756-768.e7 PubMed ID  31350118
Abstract Text  5copies per cell at steady-state. Mature microRNAs are produced within minutes, revealing tight intracellular coupling of biogenesis that is selectively disrupted by pre-miRNA-uridylation. Control over Argonaute protein homeostasis generates a kinetic bottleneck that cooperates with non-coding RNA surveillance to ensure faithful microRNA loading. Finally, regulated small RNA decay enables the selective rapid turnover of Ago1-bound microRNAs, but not of Ago2-bound small interfering RNAs (siRNAs), reflecting key differences in the robustness of small RNA silencing pathways. Time-resolved small RNA sequencing opens new experimental avenues to deconvolute the timescales, molecular features, and regulation of small RNA silencing pathways in living cells. Doi  10.1016/j.molcel.2019.06.018
Issue  4 Month  08

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