help  | about  | cite  | software

Publication : Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase.

First Author  Page Andrew R Year  2005
Journal  EMBO J. Volume  24
Pages  4304-15 PubMed ID  16369566
Abstract Text  The highly condensed chromosomes and chromosome breaks in mitotic cells of a Drosophila mutant, spotted-dick/pita, are the consequence of defects in DNA replication. Reduction of levels of Spotted-dick protein, by either RNAi or mutation, leads to the accumulation of cells that have DNA content intermediate to 2N and 4N in proliferating tissues and also compromises endoreduplication in larval salivary glands. The Spotted-dick Zinc-finger protein is present in the nuclei of cells committed to proliferation but necessary in cells undertaking S phase. We show that Spotted-dick/Pita functions as a transcription factor and that, in cultured S2 cells, it is an activator of expression of some 30 genes that include the Orc4 gene, required for initiation of DNA replication. Chromatin immunoprecipitation indicates that it associates with the genes that it activates in S2 cells together with other sites that could represent genes activated in other tissues. We discuss the role of Spotted-dick in the coordination of cellular growth and DNA replication. Doi  10.1038/sj.emboj.7600890
Issue  24 Month  Dec

Publication Annotations Displayer

102 Entities

43 Mesh Terms