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Publication : Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.

First Author  Ni Jian-Quan Year  2008
Journal  Nat. Methods Volume  5
Pages  49-51 PubMed ID  18084299
Abstract Text  The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method. Doi  10.1038/nmeth1146
Issue  1 Month  Jan

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