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Publication : Drosophilamotor neurons.

First Author  Brown Jeremy R Year  2019
Journal  Biol Methods Protoc Volume  4
Pages  bpz010 PubMed ID  31403085
Abstract Text  DrosophilaCNS and NMJ that allows processing of multiple genotypes within a single staining tube. We also present a computer script called Automated Image Analysis with Background Subtraction which facilitates identification of motor nuclei, quantification of pixel intensity, and background subtraction. Together, these techniques provide a pipeline for neuroscientists to compare levels of different biomolecules in motor nuclei. We conclude that these methods should be adaptable to a variety of different cell and tissue types for the improvement of efficiency, reproducibility, and throughput during data quantification. Doi  10.1093/biomethods/bpz010
Issue  1

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