We will first introduce the use of the Query Builder by altering a template query. We will start with the template 'For the following gene symbol, show its gene identifier' and alter it to collect all gene identifiers, symbols and FBgns from genes on the 4th chromosome of Drosophila melanogaster.

Find the template 'Gene symbol --> Gene identifier' and run the query for the default Drosophila gene.

Change the query so that it also gives you the FBgn number:

• Click on 'Query' in the trail (near top of results page) to return to the template query form. Here, click on 'Edit Query' to display this query in the Query Builder. - Note that 'identifier' and 'symbol' in the left column have a blue background to indicate that they are selected for output.

• To make the FBgn number appear on the result page as well, click on 'SHOW' next to 'organismDbId' under 'Gene'. 'organismDbId' will now appear in blue and can be found in the output section below. The order of the columns can be changed by clicking and dragging the output boxes.



• Scroll down and click 'Show results' to run the query again.