help  | about  | cite  | software

Publication : A dicentric chromosome of Drosophila melanogaster showing alternate centromere inactivation.

First Author  Agudo M Year  2000
Journal  Chromosoma Volume  109
Pages  190-6 PubMed ID  10929197
Abstract Text  Dicentric chromosomes are rarely found, because they interfere with normal cell division causing chromosome instability. By in situ hybridization of region-specific heterochromatic yeast artificial chromosomes we have found that the artificially generated C(1)A chromosome of Drosophila melanogaster has two potential centromeres: one carries all the sequences of the centromere of the Y chromosome and the other carries only a part of the Y centromeric region that is rich in telomere-related sequences. Immunostaining with anti-Bub1 (a kinetochore-specific marker) shows that, in spite of the differences in sequence, both centromeres can be active although as a rule only one at a time. In a small fraction of the chromosomes centromere inactivation is incomplete, giving rise to true dicentric chromosomes. The centromere inactivation is clonally inherited, providing a new example of epigenetic chromosome imprinting and the possibility of genetically dissecting this process. The involvement of telomere-related sequences in centromere function is discussed. Issue  3
Month  Jun

Publication Annotations Displayer

12 Entities

13 Mesh Terms