help  | about  | cite  | software

Publication : Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila.

First Author  Yamasaki Yasutoyo Year  2006
Journal  Dev. Growth Differ. Volume  48
Pages  411-8 PubMed ID  16961588
Abstract Text  Mi-2, the central component of the nucleosome remodeling and histone deacetylation (NuRD) complex, is known as an SNF2-type ATP-dependent nucleosome remodeling factor. No morphological mutant phenotype of Drosophila Mi-2 (dMi-2) had been reported previously; however, we found that rare escapers develop into adult flies showing an extra bristle phenotype. The dMi-2 enhanced the phenotype of ac(Hw49c), which is a dominant gain-of-function allele of achaete (ac) and produces extra bristles. Consistent with these observations, the ac-expressing proneural clusters were expanded, and extra sensory organ precursors (SOP) were formed in the dMi-2 mutant wing discs. Immunostaining of polytene chromosomes showed that dMi-2 binds to the ac locus, and dMi-2 and acetylated hisotones distribute on polytene chromosomes in a mutually exclusive manner. The chromatin immunoprecipitation assay of the wing imaginal disc also demonstrated a binding of dMi-2 on the ac locus. These results suggest that the Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation. Doi  10.1111/j.1440-169X.2006.00880.x
Issue  7 Month  Sep

Publication Annotations Displayer

2 Entities

19 Mesh Terms